Learning How to Identify & Sequence Proteins by Mass Spectrometry

In 1992 a mass spectrometry group was established under the leadership of Matthias Mann. In the following years we explored how to sequence peptides and identify proteins using mass spectrometers in the most sensitive way. There were three important components to this
  • the nano-electrospray ion source (figure 1 - 4)

  • the sequence tag based protein identification algorithm (slide 5)

  • protocols for gel staining, in-gel digestion and sample preparation for the analysis with mass spectrometry