Functional Proteomics

One of the early important decisions in 1999 was to dedicate all available resources to the support of the “Tandem Affinity Purification” (TAP) method [1]. The TAP method had been developed in Bertrand Seraphin’s group. It was a tag based, gentle, specific and in-vivo protein complex purification method established in yeast cells (slide 2, 3). This technology could evolve together with mass spectrometry based protein identification into a key component of an organism wide functional proteomics projects - a systematic effort to place proteins into their functional cellular context (slide 4, 6) [2].
Later we demonstrated how the TAP method could be used with cells from higher eukaryotes, the iTAP project (slide 7, 8, 9) [3].