Bioanalytical Research Group

Membrane based Protein Complexes 

The question arose whether the preparation technique is gentle enough to allow for protein complex assembly. To test this experimentally listeriolysin O was sprayed onto a bi-layer membrane. Listeriolysin O is a soluble protein that assembles on membranes in a non-covalent 30mer that inserts into the membrane and forms a large pore. 

Transmission electron microscopic picture of an extended listeriolysin pores containing membrane. Platinum shadowing was used to generate contrast.