Bioanalytical Research
Bioanalytical Research Group
MALDI Matrices
Table 1. Commonly used MALDI matrices for analysis of peptides, proteins, carbohydrates, and nucleic acids using 337 nm or 355 nm UV lasers. All matrices can be used for sample preparation using the Dried Droplet Method whereas only matrices soluble in organic solvent can be used in the Fast Evaporation Method. Matrix materials can be obtained from Aldrich and Sigma.
Matrix
Peptide / Protein
Carbohydrate
Nucleic Acid
Ref
HCCA (4-hydroxy-a-cyanocinnamic acid)
+++
1
inapinic acid (3,5-dimethoxy-4-hydroxycinnamic acid)
+++
2
DHB (2,5-dihydroxybenzoic acid)
+++
+++
3,4
THAP (2,4,6-trihydroxyacetophenone)
++
+++
5
3-hydroxy-picolinic acid
+++
6
Ferulic acid (4-hydroxy-3-methoxycinnamic acid)
+
++
2
References
1. Beavis, R. C., Chaudhary, T., and Chait, B. T. (1992) Org. Mass Spectrom. 27, 156-158.
2. Beavis, R. C., and Chait, B. T. (1989) Rapid Commun. Mass Spectrom. 3, 432-435.
3. Strupat, K., Karas, M., and Hillenkamp, F. (1991) Int. J. Mass Spectrom. Ion Proc. 111, 89-102.
4. Stahl, B., Steup, M., Karas, M., and Hillenkamp, F. (1991) Anal. Chem. 63, 1463-1466.
5. Pieles, U., Zurchner, W., Schar, M., and Moser, H. E. (1993) Nucleic Acids Res. 21, 3191-3196.
6. Wu, K. J., Steding, A., and Becker, C. H. (1993) Rapid Commun. Mass Spectrom. 7, 142-146.