Bioanalytical Research Group

iTAP Based Protein Complex Purification

pBSactTAP-C-terminal

Sequence between the T3 and T7 Primer:

GGGAGTACACTCTTCATGGCGATATACAAGACACACACAAGCACGAACACCCAGTTGCGGAGGAAATTCTCCGTAA ATGAAAACCCAATCGGCGAACAATTCATACCCATATATGGTAAAAGTTTTGAACGCGACTTGAGAGCGGAGAGCATT GCGGCTGATAAGGTTTTAGCGCTAAGCGGGCTTTATAAAACGGGCTGCGGGACCAGTTTTCATATCACTACCGTTTG AGTTCTTGTGCTGTGTGGATACTCCTCCCGACACAAAGCCGCTCCATCAGCCAGCAGTCGTCTAATCCAGAGACCC CGGATCCACATATGACCATGGCTGCACAATTGCGCGGATCCGCGATGGAAAAGAGAAGATGGAAAAAGAATTTCAT AGCCGTCTCAGCAGCCAACCGCTTTAAGAAAATCTCATCCTCCGGGGCACTTGATTATgATATTCCAACTACTGCTAG CGAGAATTTGTATTTTCAGGGTGAGCTCAAAACCGCGGCTCTTGCGCAACACGATGAAGCCGTGGACAACAAATTCA ACAAAGAACAACAAAACGCGTTCTATGAGATCTTACATTTACCTAACTTAAACGAAGAACAACGAAACGCCTTCATCC AAAGTTTAAAAGATGACCCAAGCCAAAGCGCTAACCTTTTAGCAGAAGCTAAAAAGCTAAATGATGCTCAGGCGCCG AAAGTAGACAACAAATTCAACAAAGAACAACAAAACGCGTTCTATGAGATCTTACATTTACCTAACTTAAACGAAGAAC AACGAAACGCCTTCATCCAAAGTTTAAAAGATGACCCAAGCCAAAGCGCTAACCTTTTAGCAGAAGCTAAAAAGCTA AATGATGCTCAGGCGCCGAAAGTAGACGCGAATTCCGCCGGGAAGTCAACCTGAGATCCTAGAGGGCCCTATTCTA TAGTGTCACCTAAATGCTAGAGCTCGCTGATCAGCCTCGACTGTGCCTTCTAGTTGCCAGCCATCTGTTGTTTGCCC CTCCCCCGTGCCTTCCTTGACCCTGGAAGGTGCCACTCCCACTGTCCTTTCCTAATAAAATGAGGAAATTGCATCGC ATTGTCTGAGTAGG

TAP-tag sequence and polylinker:

GCACATATGACCATGGCTGCACAATTGCGCGGATCCGCGATGGAAAAGAGAAGATGGAAAAAGAATTTCATAGCCG TCTCAGCAGCCAACCGCTTTAAGAAAATCTCATCCTCCGGGGCACTTGATTATGATATTCCAACTACTGCTAGCGAG AATTTGTATTTTCAGGGTGAGctcAAAACCGCGGCTCTTGCGCAACACGATGAAGCCGTGGACAACAAATTCAACAAA GAACAACAAAACGCGTTCTATGAGATCTTACATTTACCTAACTTAAACGAAGAACAACGAAACGCCTTCATCCAAAGT TTAAAAGATGACCCAAGCCAAAGCGCTAACCTTTTAGCAGAAGCTAAAAAGCTAAATGATGCTCAGGCGCCGAAAGT AGACAACAAATTCAACAAAGAACAACAAAACGCGTTCTATGAGATCTTACATTTACCTAACTTAAACGAAGAACAACG AAACGCCTTCATCCAAAGTTTAAAAGATGACCCAAGCCAAAGCGCTAACCTTTTAGCAGAAGCTAAAAAGCTAAATGG TGCTCAGGCGCCGAAAGTAGACGCGAATTCCGCGGGGAAGTCAACCAAGCTT

TAP-tag protein Sequence:

MEKRRWKKNFIAVSAANRFKKISSSGALDYDIPTTASENLYFQGELKTAALAQHDEAVDNKFNKEQQNAFYEILHLPNLNE EQRNAFIQSLKDDPSQSANLLAEAKKLNDAQAPKVDNKFNKEQQNAFYEILHLPNLNEEQRNAFIQSLKDDPSQSANLLAE AKKLNGAQAPKVDANSAGKSTKL